Part:BBa_K1315000

pqsR

This is the coding sequence for the transcriptional factor PqsR, which accepts PQS (Pseudomonas quinolone signal). It has been taken from Pseudomonas aeruginosa PAO1. It positively regulates the pqsABCDE promoter (BBa_K1315001), but we did not manage to confirm it.

Usage and Biology

The Pseudomonas quinolone signal (PQS) as well as its precursor 2-heptyl-4-quinolone (HHQ) are very important in mechanisms controlling virulence in Pseudomonas aeruginosa. P. aeruginosa has a key role in Cystic Fibrosis (CF). PQS can initiate signalling for various processes like virulence, motility, and biofilm formation. A good number of these processes are facilitated by PqsR, which is a transcriptional factor. An activated PqsR will activate the respective promoters, which in our project was pqsABCDE (BBa_K1315001).

http://www.ncbi.nlm.nih.gov/pubmed/21539583 http://www.ncbi.nlm.nih.gov/pubmed/?term=pqsr-dependent+and+pqsr-independent+regulation+of+motility+and+biofilm+formation+by+pqs http://www.ncbi.nlm.nih.gov/pubmed/12393198

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 461
  Illegal BamHI site found at 77
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 688
  Illegal NgoMIV site found at 909
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 645