Plasmid_Backbone

Part:BBa_K1314015

Designed by: Seak Chi U, Chan Wai Lun, CHIU, Yee Ting   Group: iGEM14_Hong_Kong-CUHK   (2014-10-07)

Homologous stable integration for nifH promoter T7 expression system Tookit

Homologous stable integration for nifH promoter T7 expression system Tookit


Usage and Biology

EcoRI PstI double digest check gel photo:

The gel photos here show an expected band size of 2600bp


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2742
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2748
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2742
    Illegal BamHI site found at 2588
    Illegal XhoI site found at 1387
    Illegal XhoI site found at 2413
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2742
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2742
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2757
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 223
    Illegal AgeI site found at 335
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None