Coding
ColOx
Part:BBa_K1313000
Designed by: Eddie Cano Group: iGEM14_ITESM-CEM (2014-10-11)
Cholesterol Oxidase
This sequence codifies an enzyme, Cholesterol Oxidase. This enzyme transforms the 3-OH group from the cholesterol into 3-keto; this reaction is not directly related with the elimination of the oxidation in the 7 position of the 7-ketocholesterol molecule. However, it is an "activation" reaction that unleashes the following reactions of the synthetic metabolic pathway, this occurs at the beginning of the degradation of sterols of many microbial pathways. The first step of this reactions is the oxidation of 3B-OH group, catalyzed by extracellular cholesterol oxidase.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 147
Illegal NgoMIV site found at 244
Illegal NgoMIV site found at 391
Illegal NgoMIV site found at 928
Illegal NgoMIV site found at 1701
Illegal AgeI site found at 520
Illegal AgeI site found at 1390 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/enzyme
None |