Coding
ColOx

Part:BBa_K1313000

Designed by: Eddie Cano   Group: iGEM14_ITESM-CEM   (2014-10-11)


Cholesterol Oxidase

This sequence codifies an enzyme, Cholesterol Oxidase. This enzyme transforms the 3-OH group from the cholesterol into 3-keto; this reaction is not directly related with the elimination of the oxidation in the 7 position of the 7-ketocholesterol molecule. However, it is an "activation" reaction that unleashes the following reactions of the synthetic metabolic pathway, this occurs at the beginning of the degradation of sterols of many microbial pathways. The first step of this reactions is the oxidation of 3B-OH group, catalyzed by extracellular cholesterol oxidase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 147
    Illegal NgoMIV site found at 244
    Illegal NgoMIV site found at 391
    Illegal NgoMIV site found at 928
    Illegal NgoMIV site found at 1701
    Illegal AgeI site found at 520
    Illegal AgeI site found at 1390
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
Parameters
None