Plasmid

Part:BBa_K1312004

Designed by: Yutaka Murata   Group: iGEM14_Kyoto   (2014-10-04)


pSB1C3 plus 6x His tag

This part is pSB1C3 which introduced 6xHis tag and stop codon UAA after Biobrick Suffix. Insert promoter,RBS and coding protein gene which is from start codon to before stop codon, if you want to add 6xHis tag to protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2070
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2070
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2076
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2070
    Illegal XhoI site found at 1054
    Illegal XhoI site found at 1946
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2070
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2070
    Illegal XbaI site found at 2085
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None