Generator
Part:BBa_K1312000
Designed by: Tatsui yusuke Group: iGEM14_Kyoto (2014-09-30)
generate some magnetosome proteins
If you use this part, you can generate magnetotactic bacteria AMB-1's magnetosome protein mamL, mamQ, mamB. These proteins are suggested that they are essential to magnetosome formation in AMB-1. We added 6X histidine tag to mamL and mamQ in order to identify these protein by western blotting. MW of mamB and mamQ are similar so we didn't add his tag to mamB.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 8
Illegal NheI site found at 31 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1608
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1073
Illegal NgoMIV site found at 1399
Illegal NgoMIV site found at 2007
Illegal AgeI site found at 1665 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 271
[edit]
Categories
Parameters
n/a | generate some magnetosome proteins |