Generator

Part:BBa_K1312000

Designed by: Tatsui yusuke   Group: iGEM14_Kyoto   (2014-09-30)


generate some magnetosome proteins

If you use this part, you can generate magnetotactic bacteria AMB-1's magnetosome protein mamL, mamQ, mamB. These proteins are suggested that they are essential to magnetosome formation in AMB-1. We added 6X histidine tag to mamL and mamQ in order to identify these protein by western blotting. MW of mamB and mamQ are similar so we didn't add his tag to mamB.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 8
    Illegal NheI site found at 31
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1608
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1073
    Illegal NgoMIV site found at 1399
    Illegal NgoMIV site found at 2007
    Illegal AgeI site found at 1665
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 271


[edit]
Categories
Parameters
n/agenerate some magnetosome proteins