Composite

Part:BBa_K1311006

Designed by: Xinye Wang   Group: iGEM14_Tsinghua-A   (2014-10-09)


K081005+TetR+B0015+pTet

Users can insert their genes downstream the pTet. In this way, people can regulate their gene expression by adding tetracycline or its analog, aTc. Since that pTet is constitutively suppressed by TetR and TetR inhibition is blocked by tetracycline or its analog, aTc.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None