Composite

Part:BBa_K1311005

Designed by: Xinye Wang   Group: iGEM14_Tsinghua-A   (2014-10-09)


K081005+TetR+B0015

Users can use this parts to regulate the expression of a specific gene if there exists a pTet promoter in the upstream of this gene. Add tetracycline or its analog, aTc into the bacteria culture to suppress the function of TetR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None