Coding

Part:BBa_K1311002:Design

Designed by: Yao Xiao   Group: iGEM14_Tsinghua-A   (2014-10-10)


left arm->TALE03->right arm


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4
    Illegal PstI site found at 154
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 17
    Illegal NheI site found at 40
    Illegal PstI site found at 154
    Illegal NotI site found at 458
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 77
    Illegal BamHI site found at 3419
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4
    Illegal PstI site found at 154
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4
    Illegal PstI site found at 154
    Illegal NgoMIV site found at 476
    Illegal NgoMIV site found at 518
    Illegal NgoMIV site found at 2752
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Considering the further assembly, the left and right arm of TALE should not be ignored. Besides, tale NTD and CTD are necessary.


Source

We chose the native TALE AvrBs3 as a scaffold for customized assembly of TALE constructs.

References