Composite

Part:BBa_K1300010

Designed by: Katty Wu   Group: iGEM14_StuyGem_NYC   (2014-06-19)

cI repressible promoter + translational ccdB

The Cubitus interruptus (CI) repressible promoter controls for the expression of ccdB. ccdB is a toxin that inhibits the function of DNA gyrase and interferes with helicase activity. As a result, processes such as DNA replication and RNA transcription are hindered, ultimately causing cell death.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 287


[edit]
Categories
Parameters
None