Composite

Part:BBa_K1300009

Designed by: Ellen Jorgensen, Katty Wu   Group: iGEM14_StuyGem_NYC   (2014-06-19)

araC gene + pBAD + cI repressor

A promoter for araC (pAraC) initiates the expression of the protein araC. On its own, araC is a repressor for pBAD promoter, preventing the expression of Cubitus interruptus (CI), which is a transcription factor that would repress the pCI promoter. However, if L (+) arabinose is present, araC will bind to the L (+) arabinose molecules instead and not repress the pBAD promoter. As a result, CI will be expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2225
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2165
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1879
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None