Composite

Part:BBa_K1300004

Designed by: StuyGem   Group: iGEM14_StuyGem_NYC   (2014-06-16)

Constitutive T7 promoter + Lock (crRNA) + coding GFP

The T7 promoter is a constitutive promoter that produces high levels of transcription in E. coli. As a result, a cis-repressed mRNA (crRNA) of GFP is constantly produced. Without the trans-activating sequence (which is coded by part BBa_J01008) that codes for trans-activating RNA (taRNA), the crRNA will remain inactive. This is because the mRNA of the cis-sequence is complementary to the RBS in mRNA. As a result, a stem-loop formation occurs and blocks off the RBS so that ribosomes can no longer dock onto the mRNA. As a result, GFP expression will not occur. However, if taRNA is present, then the RBS is freed up and GFP expression can occur once again. Please note that this construct does not code for taRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 766


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Categories
Parameters
None