Regulatory

Part:BBa_K1300002

Designed by: Katty Wu, Ellen Jorgensen   Group: iGEM14_StuyGem_NYC   (2014-06-16)

Constitutive T7 promoter + Lock (crRNA)

This is the cis-repressive sequence that is downstream of a T7 promoter, which is a constitutive promoter that produces high levels of transcription in E. coli. A gene can be placed downstream of this part so that cis-repressed mRNA (crRNA) of the gene is constantly produced. However, the gene will not be expressed into proteins because the cis-sequence of the mRNA is complementary to the RBS. As a result, a stem loop formation occurs and the RBS is blocked off, preventing protein expression from occurring.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None