Composite
Part:BBa_K1298005
Designed by: CoBRA Team Group: iGEM14_CoBRA (2014-06-19)
PgeChia 1-2 (insert) using J04500
This part contains an RBS (BBa_B0034), a promoter (lacI inducible; BBa_R0010) and a coding region (PgeChia 1-2; BBa_K1298002). It is in a pSB1C3 backbone. With this, the chitinase protein is created. Chitinase breaks down chitin, a substance found in the exoskeleton of arthropods (spiders, insects, etc.) and in the cell walls of fungi. It should be noted that there is no secretory tag, meaning that the chitinase protein is not being secreted outside of the cell, but staying inside. The part should be used to have the chitinase protein break down chitin.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 812
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 722
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 793
Illegal BsaI site found at 1044
[edit]
Categories
Parameters
None |