Composite

Part:BBa_K1298003

Designed by: CoBRA Team   Group: iGEM14_CoBRA   (2014-06-17)

PcChia 1-1 (insert) using J04500

This part contains an RBS (BBa_B0034), a promoter (lacI inducible; BBa_R0010) and a coding region (PcChia 1-1; BBa_K1298000). It is in a pSB1C3 backbone. With this, the chitinase protein is created. Chitinase breaks down chitin, a substance found in the exoskeleton of arthropods (spiders, insects, etc.) and in the cell walls of fungi. It should be noted that there is no secretory tag, meaning that the chitinase protein is not being secreted outside of the cell, but staying inside. The part should be used to have the chitinase protein break down chitin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 745
    Illegal AgeI site found at 671
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 229


[edit]
Categories
Parameters
None