Coding

Part:BBa_K1298001

Designed by: CoBRA Team   Group: iGEM14_CoBRA   (2014-06-10)

PgeChia 1-1

This is a Class I chitinase cloned from an interior spruce (Picea glauca x engelmannii). The chitinase breaks down chitin, a substance found in the exoskeleton of arthropods (spiders, insects, etc.) and in the cell walls of fungi. The part can be used to produce the chitinase protein and break down chitin. It might be useful in creating a biobrick that would be used to affect the chitin in arthropods or fungi.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 70
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 596
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 456
    Illegal NgoMIV site found at 625
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 770


[edit]
Categories
Parameters
None