Part:BBa_K1297000
Klebsiella oxytoca Urease
This part is complex of pSB1C3 vector and urease gene from Klebsiella oxytoca KCTC 1686.
Our main purpose is prevention of desertification by biocementation, and we used this enzyme for biocementation. A function of urease is catalyzing the hydrolysis of urea to release ammonia and carbon dioxide. The released carbon dioxide dissolves in water, and is precipitated with Ca2+, forming CaCO3 (biocement).
In our study, we cloned this enzyme with pBAD vector by ligation independent cloning (LIC) for enzyme overexpression, and observed the catalytic activity of it in Escherichia coli by pH indicator assay. To verify microbial biocementation, we performed a biocementation test with synthetic E. coli and detected the whole-cell activity by measuring the radius of precipitated biocement.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 225
Illegal XhoI site found at 337 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 452
Illegal NgoMIV site found at 673
Illegal NgoMIV site found at 1339
Illegal NgoMIV site found at 1754
Illegal NgoMIV site found at 1841
Illegal NgoMIV site found at 2788
Illegal NgoMIV site found at 3578 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1900
Illegal BsaI.rc site found at 148
Illegal BsaI.rc site found at 3197
Illegal SapI.rc site found at 3938
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