Part:BBa_K129004
LamB_HP-metal binding domain in LamB-153 residue
LamB tolerates insertions of long heterologous peptides at a permissive loop (between structural codons 153 and 154) exposed to the external medium without a loss of function.protein. The histidine-rich sequence Gly-His-His-Pro-His-Gly employed in LamB and called HP. HP represents one to three multiple repeats along the C-terminal part of the human plasma metal transport protein known as the histidine rich-glycoprotein(HRG. The HRG binds heme and various divalent heavy metal ions with the following apparent order of affinity: Cu21; Hg21 . Zn21 . Ni21 . Cd21 . Co21 . The HP sequence is believed to form surface metal binding sites (MBSs) of HRG, and it has been also successfully used to immobilize Cu21 and Zn21 on IMAC columns.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1316 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal SpeI site found at 1317
Illegal PstI site found at 1331
Illegal NotI site found at 7
Illegal NotI site found at 1324 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1317 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 1317
Illegal PstI site found at 1331
Illegal AgeI site found at 98
Illegal AgeI site found at 1193 - 1000COMPATIBLE WITH RFC[1000]
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