Coding

Part:BBa_K129002

Designed by: Burak YILMAZ   Group: iGEM08_METU_Turkey   (2008-11-04)


Br-bacteriorhodopsin-light driven pump

Bacteriorhodopsin (bR) expressed in purple membrane of Halobacterium salinarum is an outward light-driven proton pump, bR is excited and relaxed to the original bR via a variety of photo-intermediates. During this linear and cycling photochemical reaction (photocycle), bR transports one proton from the cytoplasm to the extracellular medium

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 810
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 95
    Illegal SpeI site found at 811
    Illegal PstI site found at 825
    Illegal NotI site found at 7
    Illegal NotI site found at 789
    Illegal NotI site found at 818
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 811
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 811
    Illegal PstI site found at 825
    Illegal NgoMIV site found at 777
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None