Composite

Part:BBa_K1290001

Designed by: Beril Gurdap   Group: iGEM14_NGSS_TR   (2014-06-20)


OmpA-SpeB_Cleavage_Site

Construct is consisting of a cell wall protein, linker sequence and SpeB cleavage site is designed. The cell wall protein OmpA is preferred, due to its abundant usage. The linker segregates the OmpA and SpeB cleavage site and thus creating space for the SpeB to access the cleavage site; the sequence of the linker is taken from the Imperial College 2010 project.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None