Part:BBa_K128008
pJK650 plasmid
Expression vector pJK650 from L. delbruckii bacteria. Easiest if the plasmid is grown up in Lactococcus lactis and extracted from there. Confers Erythromycin resistance; select in Lactococcus lactis with 5 ug/ml Em, select in Lactobacillus with 7.5 ug/ml Em.
With electroporation procedures reported by Serror et al. (App. and Env. Microbiology 2002), pJK650 is compatible with Lactobacillus delbruckii subsp. bulgaricus strains VI104 and ATCC 11842 and Lactobacillus delbruckii subsp. lactis strain ATCC 10697. Transformation efficiency ~ 10^2 transformants/ug.
For detailed electroporation protocols, please visit the MIT 2008 iGEM wiki or the Lactobacillus protocols section on openwetware.org
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 7384
Illegal XbaI site found at 7337
Illegal XbaI site found at 7388
Illegal SpeI site found at 944
Illegal PstI site found at 7348 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 7384
Illegal NheI site found at 1541
Illegal SpeI site found at 944
Illegal PstI site found at 7348 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 7384
Illegal BglII site found at 1554
Illegal BglII site found at 3036
Illegal BglII site found at 5286
Illegal BglII site found at 5442
Illegal BglII site found at 6134
Illegal BamHI site found at 7353
Illegal XhoI site found at 155
Illegal XhoI site found at 1502
Illegal XhoI site found at 2621
Illegal XhoI site found at 2714
Illegal XhoI site found at 4370
Illegal XhoI site found at 4769
Illegal XhoI site found at 5043 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 7384
Illegal XbaI site found at 7337
Illegal XbaI site found at 7388
Illegal SpeI site found at 944
Illegal PstI site found at 7348 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 7384
Illegal XbaI site found at 7337
Illegal XbaI site found at 7388
Illegal SpeI site found at 944
Illegal PstI site found at 7348 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5956
Illegal BsaI.rc site found at 2181
Illegal BsaI.rc site found at 2866
Illegal BsaI.rc site found at 6796
Illegal SapI.rc site found at 4912
Restriction Site Map of pJK650:
None |