Part:BBa_K128007
pLEM415 plasmid
Expression vector pLEM415 with biobrick sites inserted. A shuttle vector (derived from L. reuteri - E. coli) obtained by ligating PstI-digested pLEM5 with PstI-linearized E. coli vector pBII. Able to replicate in E. Coli and species of Lactobacillus. Confers Erythromycin resistance; select in E. coli with 150 ug/ml Em, select in Lactobacillus with 7.5 ug/ml Em.
With electroporation procedures reported by Serror et al. (App. and Env. Microbiology 2002), pLEM415 is compatible with Lactobacillus bulgaricus strain VI104 and Lactobacillus delbruckii subsp. lactis strain ATCC 10697. Transformation efficiency ~ 10^3 - 10^4 transformants/ug.
For detailed electroporation protocols, please visit the MIT 2008 iGEM wiki or the Lactobacillus protocols section on openwetware.org
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 701
Illegal XbaI site found at 4102
Illegal SpeI site found at 4096
Illegal PstI site found at 707
Illegal PstI site found at 4078 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 701
Illegal NheI site found at 2678
Illegal SpeI site found at 4096
Illegal PstI site found at 707
Illegal PstI site found at 4078
Illegal NotI site found at 4108 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 701
Illegal BglII site found at 2076
Illegal BamHI site found at 4090
Illegal XhoI site found at 668 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 701
Illegal XbaI site found at 4102
Illegal SpeI site found at 4096
Illegal PstI site found at 707
Illegal PstI site found at 4078 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 701
Illegal XbaI site found at 4102
Illegal SpeI site found at 4096
Illegal PstI site found at 707
Illegal PstI site found at 4078
Illegal NgoMIV site found at 129 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 5484
Illegal SapI site found at 4401
Restriction Site Map of pLEM415:
| None |