Part:BBa_K128003

p1025

This sequence codes for a short peptide, found to competitively inhibit binding of S.mutans to the tooth surface (CG et al.; Nature Biotechnol. 1999). S.mutans takes in sugars and secretes lactic acid, causing dental cavities, so introduction of this peptide into the mouth prevents cavities. This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.

from MIT iGEM 2008: this research team has shown p1025 inhibiting binding to hydroxyapatite (synthetic teeth) through a "novel tooth binding assay". The following graph shows the S. Mutans that are not bound to hydroxyapatite cell count. When p1025 was present, there were more cells unbound in t1, meaning that fewer cells bound to the hydroxyapatite.

For more about the "novel toothbinding assay": [http://2008.igem.org/Team:MIT/Tooth_binding_assay_protocol Tooth Binding Assay]

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal suffix found in sequence at 81
  Illegal EcoRI site found at 1
  Illegal XbaI site found at 16
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1
  Illegal SpeI site found at 82
  Illegal PstI site found at 96
  Illegal NotI site found at 7
  Illegal NotI site found at 89
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 1
  Illegal suffix found in sequence at 82
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 1
  Illegal XbaI site found at 16
  Illegal SpeI site found at 82
  Illegal PstI site found at 96
  Illegal NgoMIV site found at 42
  
• 1000
  COMPATIBLE WITH RFC[1000]