Plasmid
Part:BBa_K1256013
Designed by: Pei-Hong Chen Group: iGEM14_Mingdao (2014-06-10)
pSB1C3-Plac-ccdB-Cm-LacI
ccdB coding region was amplified by PCR from BBa_K145151 and exchanged with RFP on the plasmid of BBa_K1256012.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 4045
Illegal suffix found in sequence at 676 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4045
Illegal NheI site found at 221
Illegal SpeI site found at 677
Illegal PstI site found at 691
Illegal NotI site found at 684
Illegal NotI site found at 4051 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4045
Illegal BglII site found at 1966
Illegal BamHI site found at 533
Illegal XhoI site found at 1708
Illegal XhoI site found at 3921 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 4045
Illegal suffix found in sequence at 677 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 4045
Illegal XbaI site found at 4060
Illegal SpeI site found at 677
Illegal PstI site found at 691 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 442
[edit]
Categories
Parameters
None |