Composite

Part:BBa_K1256011

Designed by: Pei-Hong Chen   Group: iGEM14_Mingdao   (2014-06-10)

pSB1C3-Plac-SS-Glucanase

Mingdao pSB1C3-Plac-SS-Glucanase.png

The [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU39070 glucanase] coding sequence (cds) of Bacillus subtilis 168 was obtained from KEGG Genes Database. The gene was cut by NheI and BamHI and ligated to the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003). The cds can be easily exchanged with other BioBrick parts with NheI/PstI and SpeI/PstI, or be considered as a standard BioBrick parts (E-X-S-P).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 290
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1025
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None