Composite

Part:BBa_K1256011

Designed by: Pei-Hong Chen   Group: iGEM14_Mingdao   (2014-06-10)

pSB1C3-Plac-SS-Glucanase

Mingdao pSB1C3-Plac-SS-Glucanase.png

The glucanase coding sequence (cds) of Bacillus subtilis 168 was obtained from KEGG Genes Database. The gene was cut by NheI and BamHI and ligated to the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003). The cds can be easily exchanged with other BioBrick parts with NheI/PstI and SpeI/PstI, or be considered as a standard BioBrick parts (E-X-S-P).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 290
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1025
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None