Composite

Part:BBa_K1256010

Designed by: Pei-Hong Chen   Group: iGEM14_Mingdao   (2014-06-10)

pSB1C3-Plac-SS-Chitinase

Mingdao pSB1C3-Plac-SS-Chitinase.png

The chitinase coding sequence (cds) of Stenotrophomonas maltophilia K279a was obtained from KEGG Genes Database. The gene was cut by NheI and NsiI and ligated to the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003) with NheI and PstI, leaving a scar of NsiI/PsiI in the end.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 476
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 290
    Illegal PstI site found at 476
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 453
    Illegal BamHI site found at 1345
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 476
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 476
    Illegal NgoMIV site found at 425
    Illegal NgoMIV site found at 464
    Illegal NgoMIV site found at 622
    Illegal NgoMIV site found at 647
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 992
    Illegal NgoMIV site found at 1112
    Illegal AgeI site found at 974
    Illegal AgeI site found at 2084
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None