Composite
Part:BBa_K1256010
Designed by: Pei-Hong Chen Group: iGEM14_Mingdao (2014-06-10)
pSB1C3-Plac-SS-Chitinase
The [http://www.genome.jp/dbget-bin/www_bget?sml:Smlt0682 chitinase] coding sequence (cds) of Stenotrophomonas maltophilia K279a was obtained from KEGG Genes Database. The gene was cut by NheI and NsiI and ligated to the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003) with NheI and PstI, leaving a scar of NsiI/PsiI in the end.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 476
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 290
Illegal PstI site found at 476 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 453
Illegal BamHI site found at 1345 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 476
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 476
Illegal NgoMIV site found at 425
Illegal NgoMIV site found at 464
Illegal NgoMIV site found at 622
Illegal NgoMIV site found at 647
Illegal NgoMIV site found at 785
Illegal NgoMIV site found at 992
Illegal NgoMIV site found at 1112
Illegal AgeI site found at 974
Illegal AgeI site found at 2084 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |