Composite

Part:BBa_K1256009

Designed by: Pei-Hong Chen   Group: iGEM14_Mingdao   (2014-06-09)

pSB1C3-Plac-SS-RNase

Mingdao pSB1C3-Plac-SS-RNase.png

The RNase coding sequence (cds) of Stenotrophomonas maltophilia K279a was obtained from KEGG Genes Database. The gene was cut by NheI and BglII and ligated to the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003) cut by NheI and BamHI. PstI site can no longer be used in this plasmid due to one more site on the gene coding region.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2117
    Illegal PstI site found at 2438
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2117
    Illegal NheI site found at 290
    Illegal PstI site found at 2438
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2117
    Illegal BamHI site found at 2718
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2117
    Illegal PstI site found at 2438
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2117
    Illegal PstI site found at 2438
    Illegal NgoMIV site found at 792
    Illegal NgoMIV site found at 1381
    Illegal NgoMIV site found at 1961
    Illegal NgoMIV site found at 2059
    Illegal NgoMIV site found at 2830
    Illegal AgeI site found at 1169
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1295
    Illegal SapI.rc site found at 2744


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