Composite
Part:BBa_K1256008
Designed by: Pei-Hong Chen Group: iGEM14_Mingdao (2014-06-09)
pSB1C3-Plac-SS-DNase
The [http://www.genome.jp/dbget-bin/www_bget?smz:SMD_4069 DNase] coding sequence (cds) of Stenotrophomonas maltophilia D457 was obtained from KEGG Genes Database. The gene was cut by NheI and BamHI and ligated to the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003). The cds can be easily exchanged with NheI and BamHI. However, PstI site can no longer be used in this plasmid due to two more sites on the gene coding region.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 467
Illegal PstI site found at 674 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 290
Illegal PstI site found at 467
Illegal PstI site found at 674 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1124
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 467
Illegal PstI site found at 674 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 467
Illegal PstI site found at 674
Illegal NgoMIV site found at 410
Illegal NgoMIV site found at 509
Illegal AgeI site found at 434
Illegal AgeI site found at 797 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 935
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Categories
Parameters
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