Composite

Part:BBa_K1256008

Designed by: Pei-Hong Chen   Group: iGEM14_Mingdao   (2014-06-09)

pSB1C3-Plac-SS-DNase

Mingdao pSB1C3-Plac-SS-DNase.png

The DNase coding sequence (cds) of Stenotrophomonas maltophilia D457 was obtained from KEGG Genes Database. The gene was cut by NheI and BamHI and ligated to the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003). The cds can be easily exchanged with NheI and BamHI. However, PstI site can no longer be used in this plasmid due to two more sites on the gene coding region.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 467
    Illegal PstI site found at 674
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 290
    Illegal PstI site found at 467
    Illegal PstI site found at 674
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1124
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 467
    Illegal PstI site found at 674
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 467
    Illegal PstI site found at 674
    Illegal NgoMIV site found at 410
    Illegal NgoMIV site found at 509
    Illegal AgeI site found at 434
    Illegal AgeI site found at 797
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 935


[edit]
Categories
Parameters
None