Part:BBa_K1256007

pSB1C3-Plac-SS-Lipase

The [http://www.genome.jp/dbget-bin/www_bget?sml:Smlt1755 lipase] coding sequence (cds) of Stenotrophomonas maltophilia K279a was obtained from KEGG Genes Database. The gene was cut by NheI and NsiI and ligated to the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003) with NheI and PstI, leaving a scar of NsiI/PsiI in the end.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 434
  Illegal PstI site found at 545
  Illegal PstI site found at 578
  Illegal PstI site found at 992
  Illegal PstI site found at 2108
  Illegal PstI site found at 2300
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 290
  Illegal PstI site found at 434
  Illegal PstI site found at 545
  Illegal PstI site found at 578
  Illegal PstI site found at 992
  Illegal PstI site found at 2108
  Illegal PstI site found at 2300
  Illegal NotI site found at 555
  Illegal NotI site found at 1494
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1017
  Illegal BamHI site found at 2098
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 434
  Illegal PstI site found at 545
  Illegal PstI site found at 578
  Illegal PstI site found at 992
  Illegal PstI site found at 2108
  Illegal PstI site found at 2300
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 434
  Illegal PstI site found at 545
  Illegal PstI site found at 578
  Illegal PstI site found at 992
  Illegal PstI site found at 2108
  Illegal PstI site found at 2300
  Illegal NgoMIV site found at 341
  Illegal NgoMIV site found at 353
  Illegal NgoMIV site found at 551
  Illegal NgoMIV site found at 839
  Illegal NgoMIV site found at 899
  Illegal NgoMIV site found at 1001
  Illegal NgoMIV site found at 1061
  
• 1000
  COMPATIBLE WITH RFC[1000]