Composite

Part:BBa_K1256006

Designed by: Pei-Hong Chen   Group: iGEM14_Mingdao   (2014-06-09)

pSB1C3-Plac-SS-Protease

Mingdao pSB1C3-Plac-SS-Protease.png

The [http://www.genome.jp/dbget-bin/www_bget?sml:Smlt0861 protease] coding sequence (cds) of Stenotrophomonas maltophilia K279a was obtained from KEGG Genes Database. The gene was cut by NheI and BamHI and ligated to the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003). The cds can be easily exchanged with NheI and BamHI. However, PstI site can no longer be used in this plasmid due to three more sites on the gene coding region.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 413
    Illegal PstI site found at 554
    Illegal PstI site found at 1739
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 290
    Illegal PstI site found at 413
    Illegal PstI site found at 554
    Illegal PstI site found at 1739
    Illegal NotI site found at 915
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2039
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 413
    Illegal PstI site found at 554
    Illegal PstI site found at 1739
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 413
    Illegal PstI site found at 554
    Illegal PstI site found at 1739
    Illegal NgoMIV site found at 518
    Illegal NgoMIV site found at 758
    Illegal NgoMIV site found at 775
    Illegal NgoMIV site found at 1019
    Illegal NgoMIV site found at 1304
    Illegal NgoMIV site found at 1339
    Illegal NgoMIV site found at 1541
    Illegal AgeI site found at 1753
    Illegal AgeI site found at 1778
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1940


[edit]
Categories
Parameters
None