Composite
Part:BBa_K1256006
Designed by: Pei-Hong Chen Group: iGEM14_Mingdao (2014-06-09)
pSB1C3-Plac-SS-Protease
The [http://www.genome.jp/dbget-bin/www_bget?sml:Smlt0861 protease] coding sequence (cds) of Stenotrophomonas maltophilia K279a was obtained from KEGG Genes Database. The gene was cut by NheI and BamHI and ligated to the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003). The cds can be easily exchanged with NheI and BamHI. However, PstI site can no longer be used in this plasmid due to three more sites on the gene coding region.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 413
Illegal PstI site found at 554
Illegal PstI site found at 1739 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 290
Illegal PstI site found at 413
Illegal PstI site found at 554
Illegal PstI site found at 1739
Illegal NotI site found at 915 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2039
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 413
Illegal PstI site found at 554
Illegal PstI site found at 1739 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 413
Illegal PstI site found at 554
Illegal PstI site found at 1739
Illegal NgoMIV site found at 518
Illegal NgoMIV site found at 758
Illegal NgoMIV site found at 775
Illegal NgoMIV site found at 1019
Illegal NgoMIV site found at 1304
Illegal NgoMIV site found at 1339
Illegal NgoMIV site found at 1541
Illegal AgeI site found at 1753
Illegal AgeI site found at 1778 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1940
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Categories
Parameters
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