Device

Part:BBa_K1256005

Designed by: Pei-Hong Chen   Group: iGEM14_Mingdao   (2014-06-09)

pSB1C3-Plac-SS-Magainin-MprF

Mingdao pSB1C3-Plac-SS-Magainin-MprF.png

MprF of Bacillus subtilis (DB2)added a RBS (BBa_B0034)was amplified by PCR and cloned into the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003) using NheI and BamHI sites. Next, the cds of magainin was synthesized on the primers and subjeted to Gibson Assembly to make this plasmid. The amino acid sequences of magainin was obtained from Sigma-Aldrich, and the nucleotide sequence was optimized for engineering Escherichia coli using the codon optimization tool provided by Integrated DNA Technologies (IDT).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 362
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2888
    Illegal BamHI site found at 2957
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1283
    Illegal NgoMIV site found at 2339
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None