Device

Part:BBa_K1256004

Designed by: Pei-Hong Chen   Group: iGEM14_Mingdao   (2014-06-09)

pSB1C3-Plac-SS-Cecropin-MprF

Mingdao pSB1C3-Plac-SS-Cecropin-MprF.png

MprF of Bacillus subtilis (DB2)added a RBS (BBa_B0034)was amplified by PCR and cloned into the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003) using NheI and BamHI sites. Next, the cds of cecropin was synthesized on the primers and subjeted to Gibson Assembly to make this plasmid. The amino acid sequences of cecropin was obtained from Sigma-Aldrich, and the nucleotide sequence was optimized for engineering Escherichia coli using the codon optimization tool provided by Integrated DNA Technologies (IDT).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 398
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2924
    Illegal BamHI site found at 2993
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 371
    Illegal NgoMIV site found at 1319
    Illegal NgoMIV site found at 2375
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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