Device

Part:BBa_K1256004

Designed by: Pei-Hong Chen   Group: iGEM14_Mingdao   (2014-06-09)

pSB1C3-Plac-SS-Cecropin-MprF

Mingdao pSB1C3-Plac-SS-Cecropin-MprF.png

MprF of Bacillus subtilis (DB2)added a RBS (BBa_B0034)was amplified by PCR and cloned into the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003) using NheI and BamHI sites. Next, the cds of cecropin was synthesized on the primers and subjeted to Gibson Assembly to make this plasmid. The [http://www.sigmaaldrich.com/catalog/product/sigma/c6830?lang=en&region=TW amino acid sequences] of cecropin was obtained from Sigma-Aldrich, and the nucleotide sequence was optimized for engineering Escherichia coli using the [http://sg.idtdna.com/CodonOpt codon optimization] tool provided by Integrated DNA Technologies (IDT).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 398
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2924
    Illegal BamHI site found at 2993
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 371
    Illegal NgoMIV site found at 1319
    Illegal NgoMIV site found at 2375
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None