Part:BBa_K1256002

pSB1C3-Plac-NB-Cm-MN

Follow the design of BBa_K1256001, more restriction enzyme sites were introduced onto the vector of pSB1C3. MfeI and NsiI sites were generated following the Cm resistance gene cassette and can be used to assemble the BioBrick parts with EcoRI and PstI. Coding sequences(cds) cloned in this region can be driven by the Cm promoter. This design is benefit for multiple parts assembly and compatible to standard BioBrick asssembly rule.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal suffix found at 1076
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3145
  Illegal NheI site found at 221
  Illegal SpeI site found at 1077
  Illegal PstI site found at 1091
  Illegal NotI site found at 1084
  Illegal NotI site found at 2228
  Illegal NotI site found at 3151
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3145
  Illegal BamHI site found at 933
  Illegal XhoI site found at 2108
  Illegal XhoI site found at 3021
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 3145
  Illegal suffix found at 1077
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 3145
  Plasmid lacks a suffix.
  Illegal XbaI site found at 3160
  Illegal SpeI site found at 1077
  Illegal PstI site found at 1091
  Illegal AgeI site found at 781
  Illegal AgeI site found at 893
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.