pConst + Riboswitch + BSMT1
BSMT1 opt. activated with a constitutive promotor, regulated a riboswitch.
BSMT1 opt. (BBa_K1255001) encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase, which is a molecule catalyzes the salicylic acid to methyl salicylate. In order to do it, there must be added 10mM of salicylic acid to produce a winter green aroma. Besides this device has a constitutive promotor (BBa_J23119), it is regulated by a RNA thermometer (BBa_K115017) that is a RBS but with a loop that unfolds at a temperature around of 32º C.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
- 21Illegal BglII site found at 474
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC