Temporary

Part:BBa_K125330

Designed by: Margaret Ruzicka   Group: iGEM08_Hawaii   (2008-08-29)


P1 Lytic Replication

The P1 lytic replication region, from Enterobacteria phage P1, contains RepL which initiates replication at origin L (interior to RepL). It is regulated by a lacI promoter. When present in a cell producing LacI, the copy number is <10 copies per cell. If IPTG is added to the media, the promoter is "un-locked", causing the plasmid to be produced at >100+ copies per cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 655
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 586
  • 1000
    COMPATIBLE WITH RFC[1000]


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