Composite

Part:BBa_K1253114

Designed by: Edward Hsieh   Group: iGEM14_TAS_Taipei   (2014-06-27)


This part is used to test for TetR half-life.

The part features LacI constitutively expressed by pCI and pLac expressing TetR/RFP. TetR/RFP then inhibits pTet, thus inhibiting GFP expression. The RFP is tagged to TetR, so the half-life of RFP would equal the half-life of TetR. By knowing the half-life of LacI and the promoter strength of pCI, TetR half-life can be deduced.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4155
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1484
    Illegal AgeI site found at 1596
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2507


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Parameters
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