Composite

Part:BBa_K1253113

Designed by: Edward Hsieh   Group: iGEM14_TAS_Taipei   (2014-06-27)


TetR half-life tester

This part is used to test TetR/RFP half-life. pLac constitutively expresses TetR/RFP, making the cell red and repressing the pTet that expresses GFP and LacI. Half-lives can be determined by looking at fluorescence patterns, which can be synchronized using IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4112
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1484
    Illegal AgeI site found at 1596
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2507


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Categories
Parameters
None