Reporter

Part:BBa_K1253100

Designed by: Phillip Teng   Group: iGEM14_TAS_Taipei   (2014-06-27)


A fusion protein of TetR and mRFP

This is a fusion protein coding region of TetR and mRFP. When the gene is expressed, both TetR and mRFP expresses the protein. The mRFP acts as a reporting mechanism to visually confirm if TetR is being expressed. When TetR is not being expressed, the mRFP is not expressed.

This part is useful instead of just having a TetR coding region because it is a way to visually confirm expression without DNA sequencing.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None