Temporary
B-H-R plas

Part:BBa_K125020

Designed by: Margaret Ruzicka   Group: iGEM08_Hawaii   (2008-10-27)


lac promoter/rbs/rep/oriV/lac promoter/rbs/aadA/oriT

This is an intermediate construction step for the construction of the broad-host-range plasmid derived from RSF1010. The coding region was inserted into pSB1A3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4657


[edit]
Categories
//plasmid/component/origin
Parameters
None