Device

Part:BBa_K123009

Designed by: Jason Gardiner   Group: iGEM08_University_of_Alberta   (2008-10-28)


lacIq->BisdB

This Construct will allow for the detection and degredation of BPA in cells already containing the ER (K123003) biobrick. It works in two systems first. Together K123003 and K123009 produce a NOT gate where when Bisphenol A (BPA), Estrogen or any other estrogen mimic is in the system the product of K123003 dimerizes and attaches to the ERE site of K123009. This stops the production of TetR (R0040) by phsically stoping the Polymerase from transcribing it. This allows the pTet(C0040)promoter to turn on the production of RFP (E1010) for detection as well as BisdA(K123000) and BisdB(K123001)which allow for the degredation of BPA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2461
    Illegal BamHI site found at 2849
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1588
    Illegal NgoMIV site found at 1986
    Illegal AgeI site found at 1365
    Illegal AgeI site found at 1477
    Illegal AgeI site found at 1906
    Illegal AgeI site found at 3258
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1779
    Illegal BsaI site found at 1815
    Illegal BsaI site found at 3068


[edit]
Categories
//function/degradation/bisphenol
Parameters
None