Device
Part:BBa_K123009
Designed by: Jason Gardiner Group: iGEM08_University_of_Alberta (2008-10-28)
lacIq->BisdB
This Construct will allow for the detection and degredation of BPA in cells already containing the ER (K123003) biobrick. It works in two systems first. Together K123003 and K123009 produce a NOT gate where when Bisphenol A (BPA), Estrogen or any other estrogen mimic is in the system the product of K123003 dimerizes and attaches to the ERE site of K123009. This stops the production of TetR (R0040) by phsically stoping the Polymerase from transcribing it. This allows the pTet(C0040)promoter to turn on the production of RFP (E1010) for detection as well as BisdA(K123000) and BisdB(K123001)which allow for the degredation of BPA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2461
Illegal BamHI site found at 2849 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1588
Illegal NgoMIV site found at 1986
Illegal AgeI site found at 1365
Illegal AgeI site found at 1477
Illegal AgeI site found at 1906
Illegal AgeI site found at 3258 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1779
Illegal BsaI site found at 1815
Illegal BsaI site found at 3068
[edit]
Categories
Parameters
//function/degradation/bisphenol
None |