Part:BBa_K123003

ER
This basic part is derived from the Human Estrogen Receptor Alpha and optimized for activity in E. coli. When estrogen or an estrogen like compound is present the receptor binds to it and forms a homodimer. This homodimer is then able to bind to an Estrogen Responsive Element (ERE BBA_K123002). In humans this estrogen receptor (ER) is a transcription factor that initiates transcription. However because we are interested in using this protein in bacteria we could not assume the same enhancer like effects. We have therefore designed this part to act as a repressor when bound to the ERE by physically blocking the polymerase.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 24
  Illegal NgoMIV site found at 902
  Illegal AgeI site found at 870
  Illegal AgeI site found at 1841
  
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

Figure 1:
This figure depicts the effect that ER has on the growth rate of a cell quantified by optical density of the culture at 600nm. Our control was a strain containing an empty I0500 plasmid. ER1 is the DNA binding domain while ER2 is the ligand binding domain. We separated these to determine the toxicity of each domain.