Project

Part:BBa_K1230016

Designed by: Andrés Ferriño Iriarte   Group: iGEM13_Ciencias-UNAM   (2013-09-26)


pLacI-rbs-MarA-rbs-GFP-double terminator-pLsrA-rbs-LL-37


General Design Our design has two systems: The Antimicrobial Peptide System, which produces the peptide LL-37; and The Resistance and Expulsion System which produces the MarA protein, which activates the arcAB & TolC operons, activating the arcAB & TolC efflux pump.

Antimicrobial Peptide System H. pylori and other pathogenic bacteria produce AI-2, a quorum sensing molecule. This signal is detected by the pLsrA promoter (BBa_K117002), which allows the transcription of LL37 (BBa_K1230001), which is traduced and then translated into the antimicrobial peptide LL37. Once LL37 is expelled by the ArcAB-TolC efflux pump, it can act against H. pylori.

Resistance & Expulsion System This system is induced by promoter LacI (BBa_R0010), which allows the transcription of marA gene (BBa_K1230000), producing the protein MarA. MarA acts on the arcAB and tolC operons, which activates de ArcAB-TolC efflux pump, allowing the expulsion of LL37 peptide.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 380
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1292


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