Composite

Part:BBa_K1222005

Designed by: Tingling Ke   Group: iGEM13_TzuChiU_Formosa   (2013-09-21)


pGFP(T7 promoter+lac operator GFP antisense 2+T7 terminator)

T7 promoter + lac operator +GFP antisense + T7 terminator.This part is IPTG inducible for regulating generation of CamR antisense.We design three GFP antisense for inhibit GFP.(BBa_K1222987,BBa_K1222986,BBa_K1222985)This part shows CamR antisense 2.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 104
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None