Composite

Part:BBa_K1222001

Designed by: Tingling Ke   Group: iGEM13_TzuChiU_Formosa   (2013-09-24)


pGFP (T7 promoter+lac operator+GFP antisense"3"+T7 terminator+Lac promoter+LacI)

This part is IPTG inducible for regulating the production of GFP antisense.We have designed four sets of GFP antisense to inhibit GFP.(BBa_K1222987, BBa_K1222986, BBa_K1222985)but we have only shown one(GFP antisense 3) of the four antisense as the other three are relatively similar just the GFP antisense is different. The GFP designed is used to test its efficiency on inhibiting(knockdown) the target genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 104
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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