Composite

Part:BBa_K122011

Designed by: David Ouyang   Group: iGEM08_Rice_University   (2008-10-25)


tRNA + ARFP

Verification of tRNA suppression/ point mutation proof of concept.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3
    Illegal suffix found in sequence at 189
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3
    Illegal SpeI site found at 190
    Illegal PstI site found at 204
    Illegal NotI site found at 9
    Illegal NotI site found at 197
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3
    Illegal suffix found in sequence at 190
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3
    Illegal XbaI site found at 18
    Illegal SpeI site found at 190
    Illegal PstI site found at 204
    Illegal AgeI site found at 854
    Illegal AgeI site found at 966
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None