Coding

Part:BBa_K1218019

Designed by: Alex Constantino   Group: iGEM13_Stanford-Brown   (2013-08-30)


CASCADE Complex

This polycistronic sequence contains the CasABCDE genes that form the CASCADE complex. Functions in the innate CRISPR-Cas immune system of Escherichia coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3094
    Illegal BglII site found at 4279
    Illegal BamHI site found at 23
    Illegal BamHI site found at 3224
    Illegal BamHI site found at 3585
    Illegal BamHI site found at 4482
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5203
    Illegal AgeI site found at 784
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1764


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Categories
Parameters
None