RNA

Part:BBa_K1218000

Designed by: Alex Constantino   Group: iGEM13_Stanford-Brown   (2013-08-16)


Minimal CRISPR Array

This part is a minimal CRISPR array that directs Cas9 binding and interference. It contains two BsaI sites for simple cloning of new targeting spacers.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 213
    Illegal BsaI.rc site found at 190


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Categories
Parameters
None