Composite

Part:BBa_K1217000

Designed by: Ma Tsz Shan   Group: iGEM13_Hong_Kong_HKU   (2013-09-15)

pT7-ppk1(T.forsythia)

Polyphosphate kinase (ppk1) (E.C. 2.7.4.1) catalyzes the formation of polyphosphate from ATP. In BBa_K1217000, ppk1 was under the control of T7 promotor. Under iptg induction, over-expression of ppk1 can reduce the phosphate level in the medium by its incorporation into polyphosphate synthesis inside the bacteria.

Usage and Biology

Testing

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 364
    Illegal BglII site found at 1826
    Illegal XhoI site found at 108
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1284
    Illegal NgoMIV site found at 1596
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 384


[edit]
Categories
Parameters
None