Acetyl esterase (aes) from Escherichia Coli with TEV and poly-HIS tags
Acetyl esterase - This is a cytosolic hydrolase that can catalyze hydrolysis of esters of p-nitrophenyl derivatives. The TEV tag can then be used to have the TEV protease specifically cleave off the poly-HIS tag from the purified protein .
A form of this protein without TEV and poly-HIS tags can be found here.
Usage and Biology
The hydrolase capacity of acetyl esterase makes it suitable for reporter application with substrates like acetylated xylan, ethyl acetate, cephalosporin C and derivatives . Further it can be used for desacteylation of β-Lactam antibiotics .
The acetyle esterase, apart from its hydrolase activity also binds competitively to malT, a transcription activator for the maltose operon, thus inhibiting regulation of genes required for maltose catabolism .
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 27
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal SapI site found at 951
The final construct was sequenced.