Coding

Part:BBa_K1212004

Designed by: Amy Soon   Group: iGEM13_UC_Davis   (2013-09-16)

TAL repressor 1

Transcription activator-like (TAL) repressor DNA binding protein that binds to a specific DNA sequence, called TALe Binding Site 1 (TBS 1) (part BBa_K1212003). As reported by Meckler et al, this TAL repressor has a low binding affinity and TALe repeat sequence shown below. [1]

TAL1repeats_UCDavis.jpg



References

[1] J. F. Meckler, M. S. Bhakta, M. S. Kim, R. Ovadia, C. H. Habrian, A. Zykovich, et al., "Quantitative analysis of TALE-DNA interactions suggests polarity effects," Nucleic Acids Res, vol. 41, pp. 4118-28, Apr 2013.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3
    Illegal BamHI site found at 2397
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 21
    Illegal NgoMIV site found at 2223
    Illegal AgeI site found at 2404
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None