DNA

Part:BBa_K1211003

Designed by: Joel Sher   Group: iGEM13_Yale   (2013-08-16)


phaC1-A-B1 [P(3HB) synthesis] under pBAD

This is the Tokyo Tech 2012 Biobrick (BBa_K934001) with the wild type promoter removed and replaced with pBAD. This makes the system inducible under the addition of arabinose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1140
    Illegal BglII site found at 1965
    Illegal BamHI site found at 237
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 446
    Illegal NgoMIV site found at 517
    Illegal NgoMIV site found at 1117
    Illegal NgoMIV site found at 1429
    Illegal NgoMIV site found at 1708
    Illegal NgoMIV site found at 2360
    Illegal NgoMIV site found at 2382
    Illegal AgeI site found at 72
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 274
    Illegal BsaI site found at 4226
    Illegal SapI site found at 54


[edit]
Categories
Parameters
None