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DNA
Part:BBa_K1211003
Designed by: Joel Sher Group: iGEM13_Yale (2013-08-16)
phaC1-A-B1 [P(3HB) synthesis] under pBAD
This is the Tokyo Tech 2012 Biobrick (BBa_K934001) with the wild type promoter removed and replaced with pBAD. This makes the system inducible under the addition of arabinose.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1140
Illegal BglII site found at 1965
Illegal BamHI site found at 237 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 446
Illegal NgoMIV site found at 517
Illegal NgoMIV site found at 1117
Illegal NgoMIV site found at 1429
Illegal NgoMIV site found at 1708
Illegal NgoMIV site found at 2360
Illegal NgoMIV site found at 2382
Illegal AgeI site found at 72 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 274
Illegal BsaI site found at 4226
Illegal SapI site found at 54
[edit]
Categories
Parameters
None |